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A new alternative to produce gibberellic acid by solid state fermentation BABT
Rodrigues,Cristine; Vandenberghe,Luciana Porto de Souza; Teodoro,Juliana; Oss,Juliana Fraron; Pandey,Ashok; Soccol,Carlos Ricardo.
Gibberellic acid (GA3) is an important hormone, which controls plant's growth and development. Solid State Fermentation (SSF) allows the use of agro-industrial residues reducing the production costs. The screening of strains (four of Gibberella fujikuoroi and one of Fusarium moniliforme) and substrates (citric pulp, soy bran, sugarcane bagasse, soy husk, cassava bagasse and coffee husk) and inoculum preparation study were conducted in order to evaluate the best conditions to produce GA3 by SSF. Fermentation assays were carried out in erlenmeyers flasks at 29°C, with initial moisture of 75-80%. Different medium for inoculum production were tested in relation to cells viability and GA3 production by SSF. F. moniliforme LPB 03 and citric pulp were chosen for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gibberellic acid; Fusarium moniliforme; Solid state fermentation; Inoculum; Agro-industrial residues.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700023
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Crude Fermented Extract Containing Gibberellic Acid Produced by Fusarium moniliforme is an Alternative to Cost Reduction in Biofactories BABT
Costa,Jefferson da Luz; Silva,André Luís Lopes da; Gollo,André Luiz; Brondani,Gilvano Ebling; Santos,Leandro Freire dos; Rodrigues,Cristine; Vandenberghe,Luciana Porto de Souza; Soccol,Carlos Ricardo.
ABSTRACT Nidularium procerum and Nidularium innocentii (Bromeliaceae) were cultivated in vitro on media supplemented with different sources and levels of GA3 (gibberellic acid). These sources were the commercial powder (analytical degree) and fermented extract obtained by Fusarium moniliforme via solid state fermentation. The in vitro elongation and rooting of these plants were evaluated after 50 days of cultivation. The GA3 present in the fermented extract possess the same effect of purified GA3 (analytical degree) for the increase of the height of aerial part of shoots of N. innocentii, but not for the N. procerum being the GA3 fermented extract in a lesser degree. The GA3 fermented extract influences negatively the rooting in N. innocentii, while GA3...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Shelf life; Bromeliad; Gibberellin; Solid state fermentation; Micropropagation; Biofactory.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100427
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Effect of caffeine and tannins on cultivation and fructification of Pleurotus on coffee husks BJM
Fan,Leifa; Soccol,Andréa Thomaz; Pandey,Ashok; Vandenberghe,Luciana Porto de Souza; Soccol,Carlos Ricardo.
The objective of this work was to study the effect of caffeine and tannins on Pleurotus sp. cultivation and to evaluate the feasibility of using coffee husks as substrate for mushroom cultivation. Eight strains of P. ostreatus and two strains of P. sajor-caju were screened on a medium prepared from agar extract of coffee husk. Based on best mycelial growth and biomass production, the strain P. ostreatus LPB 09 was selected for detailed studies. With the increase of caffeine concentration, the mycelial growth and the biomass production decreased, and no growth was observed when concentration of caffeine was 2500 mg/L. Furthermore, Pleurotus did not degrade the caffeine, but absorbed it. Tannin under 100 mg/L in the medium stimulated the growth of mycelia,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cultivation; Pleurotus; Caffeine; Tannin; Detoxification.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400003
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Evaluation of probiotic properties of Pediococcus acidilactici B14 in association with Lactobacillus acidophilus ATCC 4356 for application in a soy based aerated symbiotic dessert BABT
Ribeiro,Maria Carolina de Oliveira; Vandenberghe,Luciana Porto de Souza; Spier,Michele Rigon; Paludo,Katia Sabrina; Soccol,Carlos Ricardo; Soccol,Vanete Thomaz.
The aim of this study was to evaluate the probiotic properties of Pediococcus acidilactici B14 and to study its resistance in the gastrointestinal system when combined with Lactobacillus acidophilus ATCC 4356 and used in a potentially symbiotic aerated soy based dessert. P. acidilactici B14 showed some important probiotic characteristics such as survival rate of 45.9% at pH 2.5; 72.4% in 0.3% bile salts and 95.8% after gastrointestinal transit at pH 4.0. Tolerance against the antibiotics cephalexin, neomycin, vancomycin, cefotaxime and penicillin G was also observed. The strain inhibited antagonism against the following cultures: Escherichia coli ATCC 25922, Bacillus cereus ATCC 33018, Staphylococcus aureus ATCC 6538P and Salmonella sp. The mixed culture...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pediococcus acidilactici; Lactobacillus acidophilus; Gastrointestinal system; Probiotics; Soy.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500755
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Formulated products containing a new phytase from Schyzophyllum sp. phytase for application in feed and food processing BABT
Salmon,Denise Naomi; Piva,Luíza Cesca; Binati,Renato Leal; Vandenberghe,Luciana Porto de Souza; Soccol,Vanete Thomaz; Soccol,Carlos Ricardo; Spier,Michele Rigon.
A new formulated product containing high yield of phytase from Schizophyllum sp., an important mushroom used for medicinal studies, was developed for application in feed industries and for future use in food processing. The enzyme presented a high activity yield 55.5 U/mL and 6240 U/gds in liquid and solid formulated product, respectively. It showed a good shelf-life in concentrated product, retaining 67.8% of its activity after 60 days of storage at room temperature and 90% of the activity was maintained in the liquid formulation after the same period. Powder bioformulated product maintained 77% of its activity after two months of storage, without the addition of chemical additives, which was named as a new bioformulated product containing high quantities...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phytase; Shizophyllum sp; Downstream steps; Stability; Formulation.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600001
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Lab-Scale production of Bacillus atrophaeus' spores by solid state fermentation in fifferent types of bioreactors BABT
Sella,Sandra Regina Barroso Ruiz; Guizelini,Belquis Palácio; Vandenberghe,Luciana Porto de Souza; Medeiros,Adriane Bianchi Pedroni; Soccol,Carlos Ricardo.
Studies were conducted to evaluate Bacillus atrophaeus spores' production by solid-state fermentation (SSF) using sugarcane bagasse as support and soybean molasses as substrate at lab-scale in column bioreactors (forced aeration), plastic bags and Erlenmeyer flasks (aeration by diffusion). Different moisture contents (84%, 86% and 88%; 89%, 91% and 93%) and aeration rates (30mL/min, 45mL/min, 60mL/min and 90mL/min) were studied. The best condition for spore production (3.3x10(10) CFU.g-¹dry matter) in column bioreactor was 80% of initial humidity and no aeration. In Erlenmeyer flasks and plastic bags the best sporulation production reached 1.7 up to 4.7x10(10) CFU.g-1dry matter with 88-93% of initial moisture. The aeration rate had no significant effect on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus atrophaeus; Spores; Solid state fermentation; Column bioreactor; Biofilm.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700021
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Microscale direct transesterification of microbial biomass with ethanol for screening of microorganisms by its fatty acid content BABT
Sorgatto,Vanessa Ghiggi; Carvalho,Julio Cesar de; Sydney,Eduardo Bittencourt; Medeiros,Adriane Bianchi Pedroni; Vandenberghe,Luciana Porto de Souza; Soccol,Carlos Ricardo.
Abstract We present an improved method of direct transesterification suitable for the quantitative analysis of multiple dry samples for its fatty acid content, using a minimal amount of biomass and reactants. The method features an acid-catalyzed direct alcoholysis of microgram samples of dry biomass; the rationale behind the solvent and reagent proportions chosen is discussed. The method was validated using seven microbial strains with diverse lipid content (Saccharomyces cerevisiae, Saccharomyces boulardii, Candida tropicalis, Haematococcus pluvialis, Chlorella vulgaris, Spirulina platensis and Schizochytrium limacinum), and compared with a macroscale direct transesterification method, and with gravimetric analysis of lipids extracted with solvents. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microbial oil; Ethanolysis; FAEE; Microscale; Screening.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100401
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Relation between citric acid production by solid-state fermentation from cassava bagasse and respiration of Aspergillus niger LPB 21 in semi-pilot scale BABT
Prado,Flávera Camargo; Vandenberghe,Luciana Porto de Souza; Soccol,Carlos Ricardo.
The aim of this work was to study the relation between citric acid production and respiration of Aspergillus niger LPB 21 in solid-state fermentation of cassava bagasse. The experiments were carried out in horizontal drum bioreactor coupled with a gas chromatography system. Fermentation was conduced for 144 h with initial substrate moisture of 60% using heat-treated cassava bagasse as sole carbon source. The exhausted air from the bioreactor was analyzed for the monitoring of CO2 produced and O2 consumed in order to estimate the biomass biosynthesis by the fungal culture. The metabolic activity of A. niger growth was associated to citric acid production. The system using FERSOL software determined 4.372 g of biomass/g of consumed O2. Estimated and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citric acid; Solid-state fermentation; Cassava bagasse; Horizontal drum bioreactor; Fungus respiration.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400004
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Spore production of Beauveria bassiana from agro-industrial residues BABT
Santa,Herta Stutz Dalla; Santa,Osmar Roberto Dalla; Brand,Débora; Vandenberghe,Luciana Porto de Souza; Soccol,Carlos Ricardo.
The purpose of this work was to produce Beauveria bassiana by Solid-State Fermentation using agro-industrial residues and optimizing the cultivation conditions. Refused potatoes, coffee husks and sugar-cane bagasse were tested. The blend of refused potatoes and sugar-cane bagasse (60-40%) with particle size in the range of 0.8-2 mm was used in the fermentation experiments. In Erlenmeyer flasks the best spore production was achieved with the following conditions: incubation temperature 26º C; initial pH 6.0; inoculum concentration 10(7) spores.g-1.dw and initial moisture 75%. In the column type reactor using forced aeration under the optimized conditions, the maximum production (1.07x10(10)spores.g-1.dw) was obtained at the 10th day of fermentation. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agro-industrial residues; Beauveria bassiana; Solid-state fermentation.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400007
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Thermal characterization of partially hydrolyzed cassava (Manihot esculenta) starch granules BABT
Lacerda,Luiz Gustavo; Azevedo,Jayme Augusto Menegassi; Carvalho Filho,Marco Aurélio da Silva; Demiate,Ivo Mottin; Schnitzler,Egon; Vandenberghe,Luciana Porto de Souza; Soccol,Carlos Ricardo.
Cassava starch, partially hydrolyzed by fungal á-amylase, was characterized using thermal analysis, light microscopy and X-ray diffraction. Thermal degradation was initiated at lower degradation temperatures after enzymatic treatment and the DSC (Differential scanning calorimetry) analysis showed almost similar range of gelatinization temperature, but the enthalpies of gelatinization were quite increased for the partially hydrolyzed starch granules. The results suggested that the partial degradation of the starch granules was concentrated in the amorphous regions.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amylase; Cassava; Hydrolysis; Thermal analysis.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600016
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Xylanase production by Streptomyces viridosporus T7A in submerged and solid-state fermentation using agro-industrial residues BABT
Alberton,Luiz Romulo; Vandenberghe,Luciana Porto de Souza; Assmann,Ricardo; Fendrich,Ricardo Cancio; Rodriguéz-León,José; Soccol,Carlos Ricardo.
The study of xylanase production was conducted by Streptomyces viridosporus T7A in submerged (SmF) and solid-state fermentation (SSF), using agro-industrial residues and sub-products. Napier grass, sugarcane bagasse and soybean bran were used as carbon source, substrate/support, and nitrogen source, respectively. In SmF, Napier grass (1% v/w) supplemented with soybean bran, hydroxyethylcellulose and B complex vitamins were used. Soybean bran (1.5 % w/v), B complex vitamins (0.1%), and hydroxyethilcellulose (0.15%) led to an increase in xylanase production (23.41 U/mL). In SSF, the effects of the following parameters were studied: substrate composition (sugarcane bagasse, Napier grass and soybean bran), initial moisture, and inoculum rate. In SSF, the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces; Xylanase; Submerged fermentation; Solid-state agro-industrial fermentation; Residues.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700022
Registros recuperados: 11
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